Kinetic Analysis of β-Galactosidase Activity using the PowerWaveTM HT and Gen5TM Data Analysis Software Basic enzyme kinetic determinations
نویسنده
چکیده
Most biological processes require an enzyme to act as a catalyst in order for the reaction to take place. With the exception of catalytic RNA, all enzymes are proteins. Most importantly, enzymes are highly effective in catalyzing diverse chemical reactions in a regulated, selective, and specific manner. With a few exceptions, most reactions catalyzed by enzymes can be described satisfactorily by the Michaelis-Menten Equation (Eq. 1),
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